n0551  (New England Biolabs)

Journal: Journal of Virology

Article Title: Enterovirus 71 infection induces pyroptotic brain injury via synergistic activation of classical inflammasome and viral gasdermin D cleavage

doi: 10.1128/jvi.01860-25

Figure Lengend Snippet: EV71 induces the activation of inflammatory responses in the brain tissue of BALB/c suckling mice by activating the Caspase-1 and Caspase-11 pathways. ( A ) Western blotting of VP-1, pro-IL-1β, cleaved-IL-1β, and IL-18 proteins in the brain tissue of suckling mice (30 µg per lane). ( B ) Co-localization staining of pro-IL-1β and cleaved-IL-1β in the brain tissue of suckling mice. ( C ) Co-localization staining of VP-1 and IL-18 in the brain tissue of suckling mice. NC: normal control group; EV71: enterovirus 71 infection group; VX765: EV71 infection group treated with VX765; WED: EV71 infection group treated with Wedelolactone. Compared with the EV71 group: ### P < 0.001 (scale bar: 50 µm).

Article Snippet: Four hours prior to infection in suckling mice, the following were administered via ip: VX765 (50 μg/g, Selleckchem, USA) or Wedelolactone (30 μg/g, MCE, USA), both dissolved and diluted in DMEM medium; the control group received an equal volume of DMEM (the solvent used for diluting the inhibitors).

Techniques: Activation Assay, Western Blot, Staining, Control, Infection

Journal: Journal of Virology

Article Title: Enterovirus 71 infection induces pyroptotic brain injury via synergistic activation of classical inflammasome and viral gasdermin D cleavage

doi: 10.1128/jvi.01860-25

Figure Lengend Snippet: Caspase-1 and Caspase-11 pathways jointly regulate EV71-induced pyroptosis. ( A ) Western blot of GSDMD, Caspase-11, and Caspase-1 signaling pathway proteins in the brain tissue of suckling mice (30 µg per lane). ( B ) Co-localization staining of pro-Caspase-1, cleaved-Caspase-1, in the brain tissue of suckling mice. ( C ) Co-localization staining of GSDMD, Caspase-11, in the brain tissue of suckling mice. NC: normal control group; EV71: enterovirus 71 infection group; VX765: EV71 infection group treated with VX765; WED: EV71 infection group treated with Wedelolactone (scale bar: 50 µm). Compared with the EV71 infection group: ## P < 0.01, ### P < 0.001.

Article Snippet: Four hours prior to infection in suckling mice, the following were administered via ip: VX765 (50 μg/g, Selleckchem, USA) or Wedelolactone (30 μg/g, MCE, USA), both dissolved and diluted in DMEM medium; the control group received an equal volume of DMEM (the solvent used for diluting the inhibitors).

Techniques: Western Blot, Staining, Control, Infection